pinboard March 27, 2022

  • West Seattle Blog…
  • The librarians uniting to battle school book ban laws | GMA
  • (400)
    RT @scienceisstrat1: Perhaps the most important – and terrifying – data point for humanity in recent times. This is what an emergency looks like.

    @paulg @erikbryn @dwallacewells @MichaelEMann

  • Bioinstructive implantable scaffolds for rapid in vivo manufacture and release of CAR-T cells | Nature Biotechnology
    Despite their clinical success, chimeric antigen receptor (CAR)-T cell therapies for B cell malignancies are limited by lengthy, costly and labor-intensive ex vivo manufacturing procedures that might lead to cell products with heterogeneous composition. Here we describe an implantable Multifunctional Alginate Scaffold for T Cell Engineering and Release (MASTER) that streamlines in vivo CAR-T cell manufacturing and reduces processing time to a single day. When seeded with human peripheral blood mononuclear cells and CD19-encoding retroviral particles, MASTER provides the appropriate interface for viral vector-mediated gene transfer and, after subcutaneous implantation, mediates the release of functional CAR-T cells in mice. We further demonstrate that in vivo-generated CAR-T cells enter the bloodstream and control distal tumor growth in a mouse xenograft model of lymphoma, showing greater persistence than conventional CAR-T cells. MASTER promises to transform CAR-T cell therapy by fast-tracking manufacture and potentially reducing the complexity and resources needed for provision of this type of therapy.

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